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Документ Відкритий доступ Effect of acids on biomass growth and development of Chlorella vulgaris culture(Igor Sikorsky Kyiv Polytechnic Institute, 2024) Vdovychenko, A. A.; Golub, N. B.; Zieliński, M.; Levtun, I. I.Background. Influence of dissolved nitrogen and sulfur oxides, as components of flue gases, on the cultivation of microalgae Chlorella vulgaris. Objective. To study biomass production and changes in cells of the microalgae Chlorella vulgaris with the introduction of nitrogen and sulfate acids tor simulate the effects of dissolved nitrogen and sulfur oxides, aiming to develop biotechnology for the utilization of gas emissions by microalgae. Methods. The effect of the constant introduction of small concentrations of nitrogen (up to 0.47%) and sulfate (up to 1.5%) acids on the development of Chlorella vulgaris cultures, pH changes in the cultural environment, and biomass growth were studied. Results. The utilization of gaseous emissions by Chlorella vulgaris depends on the content of nitrogen and sulfur oxides that are constantly supplied to the cultivation medium, the initial biomass concentration, and pH. It was determined that for an initial cells concentration of (85 5) 104 cells/ml in the culture medium, the threshold values of acids that do not cause significant changes in the cultivation process are 0.1% H2SO4 and 0.19% HNO3. At a sulfuric acid concentration of 0.2%, the culture cells become discolored. Conclusions. It is shown that with a constant supply of sulfuric or nitric acids above the threshold values of 0.1% H2SO4 and 0.19% HNO3, the pH of the culture medium decreases, leading to the suspension of development and the death of the Chlorella vulgaris culture. Therefore, controling these parameters will improve the ecological state of the environment and form the basis for developing biotechnology for the utilization of gas emissions by microalgae.Документ Відкритий доступ Intensification of the biohydrogen production process(Igor Sikorsky Kyiv Polytechnic Institute, 2024) Golub, N. B.; Zubchenko, L. S.; Demianenkо, I. V.; Zhang, Y.; Seminska, N. V.Background. In the last decades, humanity has faced the challenge of finding new ways to obtain renewable, environmentally friendly energy carriers. Hydrogen is one of such energy carriers; however, the current methods of its production require fossil fuels and are accompanied by significant CO2 emissions. Consequently, the energy costs needed to obtain hydrogen by electrolysis exceed the amount of energy produced by burning the hydrogen. Simultaneously, the hydrogen yields for alternative ways, such as fermentation, remain low. Objective. The aim of the work is the development of approaches to intensify the biohydrogen obtaining process from agricultural waste. Methods. An increase in hydrogen yield was achieved using specifically grown microorganisms of the Clostridium spp. A combination of the waste fermentation process with the production of hydrogen in a microbial fuel cell (MFC), which was fed with the liquid fraction after fermentation, was employed. Results. The yield of hydrogen depends on the component composition of the raw material. Higher lignin content in the raw material reduces the yield of hydrogen. The addition of Clostridium spp. to the natural consortium in the amount of 10% of the total inoculum led to an increase in hydrogen yield. The combination of two processes – fermentation and hydrogen production in a MFC – increased the yield of hydrogen by 1.7 times, along with a higher degree of organic raw materials utilization. Conclusions. The additional introduction of Clostridium spp. to the hydrogen-producing consortium leads to a 7–10% increase in the yield of hydrogen, depending on the composition of the raw material. The yield of hydrogen obtained in the fermentation process for the substrate containing corn silage is 12 +- 1% higher than for the wheat straw. In general, the combination of the fermentation and hydrogen production in the MFC in a two-stage process leads to an overall increase in the yield of hydrogen by 60 +- 5%.Документ Відкритий доступ Resistance to antibiotics and their utilization by microorganisms(Palladin Institute of Biochemistry of the NAS of Ukraine, 2021) Golub, N. B.; Shi Xinhua; Levtun, I. I.Документ Відкритий доступ Technological Solution of Biogas Output Increasing at Grain Distillery Spent Wash Fermentation(Igor Sikorsky Kyiv Polytechnic Institute, 2018) Golub, N. B.; Potapova, M. V.Документ Відкритий доступ Вплив джерела азотного живлення на приріст біомаси Chlorella vulgaris(НТУУ «КПІ», 2015) Голуб, Наталія Борисівна; Левтун, Ігор Ігорович; Тимошенко, Єлізавета Дмитрівна; Golub, N. B.; Levtun, I. I.; Tymoshenko, E. D.Документ Відкритий доступ Продукування водню при анаеробній ферментації відходів кукурудзи і соняшнику(НТУУ "КПІ", 2016) Голуб, Наталія Борисівна; Драпой, Дарина Ігорівна; Golub, N. B.; Drapoi, D. I.