2023

Постійне посилання на фонд

https://ela.kpi.ua/handle/123456789/54903

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Перегляд 2023 за Автор "Kalachinskaya, M. M."

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    Effects of metformin and preparations with pleiotropic effects on testicular biochemical indices of rats with juvenile-onset metabolic syndrome
    (Igor Sikorsky Kyiv Polytechnic Institute, 2023) Bondarenko, L. B.; Shayakhmetova, G. M.; Tkachenko, O.Ye.; Kalachinskaya, M. M.; Kovalenko, V. M.
    Background. Metabolic syndrome (MS) is a complex of disorders characterized by abdominal obesity, insulin resistance and glucose tolerance, arterial hypertension, and all types of metabolic disorders. Taking into account the wide range of symptoms accompanying MS, the use of preparations with pleiotropic effects on metabolic processes in the body could be promising for its treatment. Objective. The aim of this study is comparative estimation of metformin or its combination with vitamins' complex or liposomal preparation treatment effects on DNA, RNA, histones, ATP, ADP, AMP contents, and DNA fragmentation processes in testes of rats with MS induced in juvenile age. Methods. MS model was induced by full replacement of drinking water with 10% fructose solution in Wistar male rats of 21–23 days age (50–70 g). DNA, RNA, histones, ATP, ADP, AMP contents, and DNA fragmentation processes investigations were carried out after 60 days of MS modeling and metformin or its combination with vitamins' complex or liposomal preparation treatment. Results. In experiments with pubertal rats with MS and metformin or its combination vitamins' complex or liposomal preparation treatment, we established partially corrective effects of these medications for DNA, RNA, histones, ATP, ADP, AMP contents, and DNA fragmentation processes changes caused by MS development. Conclusions. A comparative analysis of the studied preparations' effects under MS simulation in the juvenile age showed that none of these drugs was able to completely normalize the disorders in studied indicators caused by MS. However, both combinations of metformin with vitamins' complex or liposomal preparation were still more effective in these negative changes' correction then metformin itself. Metformin with vitamins' complex caused a more pronounced influence on the processes of DNA fragmentation, the levels of adenyl nucleotides, and the energy charge of rat testicular cells, while the corrective effect of metformin with liposomal preparation was more noticeable with respect to the content of chromatin components.
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    Possibilities of Plant Preparations Use for Collagen Structure and Metabolism Disturbances Correction: Modern State of Problem
    (Igor Sikorsky Kyiv Polytechnic Institute, 2023) Bondarenko, L. B.; Kalachinskaya, M. M.; Serhiichuk, N. M.; Motronenko, V. V.; Biloshytska, O. K.
    The review summarizes information on possibilities of plant preparations use for collagen structure and me-tabolism disturbances correction. Biologically active compounds of plant origin can both stimulate and inhi-bit the biosynthesis of various types collagens, accelerate or slower down their catabolism, regulate the activi-ty of enzymes involved in the collagen's metabolism. Most of the studied compounds realize their effects si-multaneously by several mechanisms. Among them, the most common are the direct influence of the sub-stance on the processes of collagen genes expression and indirect influence via TGF-beta1- pathway. In ad-dition, a fairly common are effects on collagen synthesis by changing organism's pools of free amino acids (as the starting compounds for this protein synthesis) and by regulation of hydroxylases (performing collagen post-translational modifications and crosslinking). Besides TGF-beta1 others cytokines can also be involved in the processes of collagen metabolism regulation by compounds of plant origin. In particular, this is cha-racteristic of triterpenes and phytoestrogens. Such a variety of methods for collagens metabolism regulation creates a wide range of possibilities for developing new preparations based on extracts or pure plant com-pounds able to correct connective tissue collagen structure and metabolic disorders with minimal adverse ef-fects. Fundamentally different possibilities for the influence of plant organisms on collagens are opened with the use of genetically modified plants. Recombinant collagens allow to obtain proteins with new programmed features, making it possible to synthesize proteins with predetermined properties for medical use.