The thermal losses of a bioreactor have to be compensated for fermentation process maintaining (the temperature first of all). In order to decrease consume heat by the bioreactor and to make up thermal losses a heat insulation has been performed. In this paper a noncontact and nondestructive thermography qualification of the thermal losses and evaluation of the heat insulation’s quality for an anaerobic bioreactor is offered. By using software Researcher a thermography analysis for three different cases (without compensated heat losses; with one layer of thermal insulation; with two layers thermal insulation) is carried out. The received results show the advantages of the used approach for qualification of different kind of heat insulations.