Optimization of parameters of saline sodium citrate buffer for stability of colloidal gold nanoparticles usedin dna hybridization biosensor
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Дата
2025
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Видавець
Igor Sikorsky Kyiv Polytechnic Institute
Анотація
Background.The use of optical biosensors based on surface plasmon resonance (SPR) spectrometry have long been established as a viable alternative tothe traditional molecular biological methods, such as immu-nostainingor ELISA. Its capacity to perform real-time quantitative measurements is complemented with the possibility for the enhancement of the sensor signal with the use of optically active colloidal nanoparticles. On the other hand, few such nanoparticle-containing DNA biosensors have been developed, owing to poor colloidal stability of nanoparticles in chemical conditions suitable for hybridization of nucleic acid sequences.Objective.This study investigates the possibility of using gold nanoparticles (AuNPs) modified with thiolated oligonucleotides, 6-mercapto-1-hexanol, and lipoic acid as part of a hybridization system for biosensor de-tection of DNA sequences in order to improve its main analytical characteristics.Methods.Astudy of the colloidal stability of nanoparticlesinSSC (saline sodium citrate)media of differentmultiplicitybefore and after modification was carried out in order to select the best environment for the op-eration of the biosensor system.Aggregation of modified AuNPs was facilitated by their centrifugation, after which pelleted nanoparticles were resuspended and investigated by spectrophotometry. The conclusions re-garding the colloidal stability of AuNPs were based on the drop in concentration of colloidal AuNPs after each centrifugation.Results.The possibility of using the studied NPs in biosensor analysis was shown, and0.1SSC buffer solu-tion was determined to bethe optimal mediumfor their operational stability. Approbation of the nanoparticle-containingDNA hybridizationbiosensor based on SPR spectrometry was carried outin 2.0SSC medium.Conclusions.The prospective use ofthestudiedNPs as components of DNA hybridization systems for the detection of genetic markers has been proven
Опис
Ключові слова
surface plasmon resonance, DNA hybridization biosensors, gold nanoparticles, Philadelphia chromosome, поверхневий плазмонний резонанс, гібридизаційні ДНК-біосенсори, наночастинки золота, філадельфійська хромосома
Бібліографічний опис
Optimization of parameters of saline sodium citrate buffer for stability of colloidal gold nanoparticles usedin dna hybridization biosensor / M. S. Sobolevskyi, I. Iu. Holubiev, A. М. Lopatynskyi, A. V. Samoylov, G. V. Dorozinsky, O. M. Lyapin, R. V. Khrystosenko, V. І. Chegel, S. V. Dzyadevych, O. O. Soldatkin // Innovative Biosystems and Bioengineering : international scientific journal. – 2025. – Vol. 9, No. 2. – P. 12-20. – Bibliogr.: 25 ref.