Innovative Biosystems and Bioengineering

Постійне посилання на фонд

https://ela.kpi.ua/handle/123456789/2175

ISSN 2616-177X (Online) Періодичність: 4 рази на рік
Рік заснування: 2017
Тематика: оригінальні й оглядові статті та короткі повідомлення з проблем прикладної біології, біомедицини, біотехнології, біоінженерії, біофізики, біоінформатики та хімії живого. Журнал передбачає системний підхід для об’єднання наукової думки фахівців різної спеціалізації, які працюють із науково-технічними розробками в галузі «life sciences»
Офіційний сайт: https://ibb.kpi.ua/

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Зараз показуємо 1 - 20 з 180

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A New Algorithm to Analyze the Video Data of Cell Contractions in Microfluidic Platforms
(Igor Sikorsky Kyiv Polytechnic Institute, 2018) Nikolov, Nikolay; Visone, Roberta; Nesteruk, Igor; Rasponi, Marco; Redaelly, Alberto; Ніколов, Микола Олександрович; Візоне, Роберта; Нестерук, Ігор Георгійович; Распоні, Марко; Редаеллі, Альберто; Николов, Николай Александрович; Визоне, Роберта; Нестерук, Игорь Георгиевич; Распони, Марко; Редаелли, Альберто
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Analysis of Fractional-Order Model of COVID-19 Pandemics With a Nonlinear Incidence Rate
(Igor Sikorsky Kyiv Polytechnic Institute, 2020) Varghese, Vinod; Bhoyar, Sonal; Nisar, Kottakkaran Sooppy
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Anode biofilm formation with applied external voltage
(Igor Sikorsky Kyiv Polytechnic Institute, 2023) Koltysheva, D. S.; Shchurska, K. O.; Kuzminskyi, Ye. V.
Background. The formation of an exoelectrogenic biofilm in a microbial fuel cell (MFC) is an important stage, because it affects later on current generation by the system. The fermented residue after methanogenesis as an inoculum contains not only exoelectrogenic microorganisms, but also methanogens, which reduce the productivity of MFC. The use of current allows the formation of a biofilm enriched with exoelectrogenic microorganisms. Objective. The purpose of our study was to establish the parameters of MFC under periodic application of external voltage. Methods. A two-chamber H-type MFC with a salt bridge between the chambers was used for the study. The anolyte was stirred with a magnetic stirrer for 4 h a day and a 3V voltage was simultaneously applied to create selective conditions for exoelectrogenic biofilm growth. Results. The application of external voltage stimulated the increase in the current and voltage of the MFC. With the periodic application of an external voltage, the MFC current increased to 788 ± 40 mA for the MFC with a resistor and without load. After disconnection and discharge, the MFC current dropped to 189 ± 10 mA for the MFC without load and to 154 ± 8 mA for the MFC with a resistor, respectively. Under the conditions of MFC operation without applying external voltage, the current was 960 ± 50 mA for MFC with an open circuit and 672 ± 35 mA for MFC with a closed circuit when a resistor is connected. For all MFC, the current gradually decreased over time. MFC demonstrated capacitive behaviour: after accumulating charge for 4 h, a discharge from 622 ± 30 mV to 462 ± 23 mV was observed. Microscopy showed fouling of the anode. Since the fermented residue after methanogenesis is mixed consortium, the anodic biofilm was also mixed consortium enriched with different species of exoelectrogens. Conclusions. Periodic application of external voltage allowed to increase the current by 17% and double the voltage compared to MFC without external voltage supply. However, after disconnecting the external voltage source, the MFC gradually discharged, that is, the current and voltage decreased. The maximum value of the current of the MFC with an open circuit was 22% more than the MFC with a closed circuit.
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Antibacterial activity of 1-dodecylpyridinium tetrafluoroborate and its inclusion complex with sulfobutyl ether-B-cyclodextrin against mdr acinetobacter baumannii strains
(Igor Sikorsky Kyiv Polytechnic Institute, 2023) Rogalsky, S.; Hodyna, D.; Semenyuta, I.; Frasinyuk, M.; Tarasyuk, O.; Riabov, S.; Kobrina, L.; Tetko, I.; Metelytsia, L.
Background.The bacterial pathogen Acinetobacter baumanniiis one of the most dangerous multi-drug-resistant (MDR) microorganisms, which causes numerous bacterial infections. Nowadays, there is an urgent need for new broad-spectrum antibacterial agents with specific molecular mechanisms of action. Long-chain 1-alkylpyridinium salts are efficient cationic biocides which can inhibit enzymes involved in the biosynthesis of bacterial fatty acids.Incorporating these compounds into inclusion complexes with cyclic oligosaccharide B-cyclodextrin can reduce their relatively high acute toxicity.Objective. The aim of this research was to develop new anti-A.baumanniiagents based on hydrophobic 1-alkylpyridinium salt and its inclusion complex with sulfobutyl ether B-cyclodextrin (SBECD). Methods. Hydrophobiccationic biocide 1-dodecylpyridinium tetrafluoroborate (PyrC12-BF4) and its inclu-sion complex with SBECD have been synthesized. The structure of the SBECD/PyrC12-BF4complex was characterized by 1H Nuclear Magnetic Resonance spectroscopy, as well as UV spectroscopy. In vitroanti-bacterial activity of the synthesized compounds was estimated against MDR clinical isolates of A. baumanniiusing standard disc diffusion method. Acute toxicity studies were performed on Daphnia magnamodel hydro-biont.Molecular docking was performed using the crystal structure of the A.baumannii3-oxoacyl-[acyl-carrier-protein] reductase(FabG).
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Antifungal activity and cytotoxicity of imidazole- and morpholine-based lysosomotropic detergents
(Igor Sikorsky Kyiv Polytechnic Institute, 2025) Hodyna, D.; Kovalishyn, V.; Shulha, Yu.; Trokhimenko, O.; Aksenovska, O.; Rogalsky, S.; Metelytsia, L.
Background. The spread of infectious diseases caused by drug-resistant bacteria and fungi, especially nosocomial strains, is currently considered a serious medical problem. It is known that the fungus Candida albicans is the most common causative agent of candidal infection, including the severe type. The emergence and rapid formation of drug resistance, as a risk factor in the treatment of oncological diseases burdened by candidal infection, requires new therapeutic approaches, including the study of synthetic bioregulators with antifungal and anticancer efficacy. Objective. To synthesize and to determine the antifungal activity and cytotoxicity of imidazole- and morpholine-based lysosomotropic detergents (LDs) comprising both dodecyl radicals and ester-functionalized long alkyl chains. Methods. To develop the QSAR models by the OCHEM platform, machine learning methods such as Transformer Convolutional Neural Network (Trans-CNN), Transformer Convolutional Neural Fingerprint (TransCNF), and Random Forest (RF) were used. Imidazole- and morpholine-based lysosomotropic detergents comprising both dodecyl radicals and ester-functionalized long alkyl chains were synthesized and characterized by 1H Nuclear Magnetic Resonance spectroscopy. The antifungal properties of studied compounds were estimated by the disc diffusion method against the С. albicans ATCC 10231, С. albicans, С. glаbrata and С. krusei clinical isolates. The in vitro cytotoxic activity of LDs was evaluated by IC50 value against the throat cancer HEp-2 cell lines. AutoDock Vina software was used for the evaluation of the synthesis compounds as ligands of several antifungal targets. Results. The identified and synthesized imidazole- and morpholine-based LDs showed high antifungal potential against all Candida spp., specifically against the fluconazole-resistant С. albicans, С. glаbrata, and С. krusei clinical isolates. Imidazole-based LD 1 (IM-C12) and morpholine-based LD 4 (Mor-C12) were the most active against tested fungal strains. Molecular docking results suggest that the antifungal mechanisms of the compounds may be related to the inhibition of fungal lanosterol 14-demethylase. The cytotoxic results of the synthesized compounds against the HEp-2 cell line demonstrated that morpholine-based LDs are less cytotoxic compared to imidazole-based. Conclusions. It was found that LD IM-C12 (1) is the most promising cytostatic and antifungal agent based on the obtained results. LDs IM-CH2COOC10 (2) and Mor-C12 (4), which, under conditions of chemical modification, including through the carbon chain, may also be interesting for developing potential anticancer agents.
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Antimicrobial activity of composite preparations based on biosurfactants and heterocyclic amino-containing 1,4-naphtoquinone derivatives
(Igor Sikorsky Kyiv Polytechnic Institute, 2024) Polish, N. V.; Marintsova, N. G.; Zhurakhivska, L. R.; Komarovska-Porokhnyavets, O. Z.; Lubenets, V. I.; Pokynbroda, T. Ya.; Karpenko, О. V.
Проблематика. Розроблення високоефективних та екологічно безпечних композиційних препаратів з антимікробною активністю є актуальною проблемою біотехнології та фармації. Мета. Визначення антимікробної активності нових композиційних препаратів на основі рамноліпідів із гетероциклічними аміно- вмісними похідними 1,4-нафтохінону щодо тест-бактерій Escherichia coli B-906, Staphylococcus aureus 209-P, Mycobacterium luteum B-917 і грибів Candida tenuis UCM Y-70, Aspergillus niger UCM F-1119. Методика реалізації. Дослідження in vitro антимікробної дії гетероциклічних аміновмісних похідних 1,4-нафтохінону, біосурфак- тантів і композиційних препаратів на їх основі щодо культур бактерій Escherichia coli B-906, Staphylococcus aureus 209-P, Mycobacterium luteum B-917 і грибів Candida tenuis UCM Y-70, Aspergillus niger UCM F-1119 шляхом дифузії речовини в агар на твердому живильному середовищі та методом серійних розведень. Прогнозування токсичності гетероциклічних аміновмісних похідних 1,4-нафтохінону з використанням методології in silico за допомогою програмного забезпечення ProTox-II. Результати. Встановлено, що 2-хлоро-3-((1-метил-1H-піразол-3-іл)аміно)нафтален-1,4-діон 3.2 та 2-хлоро-3-((3-(п-толіл)-1Н- піразол-5-іл) аміно) нафтален-1,4-діон 3.3 виявляють антимікробну активність щодо тестових мікроорганізмів, а в їх композицій- них препаратах із рамноліпідами-біосурфактантами антимікробна дія підсилюється. За результатами розрахункової оцінки cпрогнозовано, що досліджені гетероциклічні аміновмісні похідні 1,4-нафтохінону можна віднести до IV класу токсичності. Висновки. Визначено, що антимікробна активність композиційних препаратів 2-хлоро-3-((1-метил-1H-піразол-3-іл)аміно)нафта- лен1,4-діону 3.2 та 2-хлор-3-(3-(3-(р-толіл)-1H-піразол-5-іл)аміно)нафталін-1,4-діону 3.3 із рамноліпідами підвищилась порівняно із синтезованими препаратами.
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Antimicrobial Activity of Fomitopsis Officinalis (Vill.) Bondartsev & Singer in Pure Culture
(Igor Sikorsky Kyiv Polytechnic Institute, 2021) Mykchaylova, O. B.; Poyedіnok, N. L.
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Antimicrobial and cytotoxic characteristics of antibiotic streptofungin
(Igor Sikorsky Kyiv Polytechnic Institute, 2023) Klochko, V. V.; Todosiichuk, T. S.; Kobzysta, O. P.; Bobyr, V. V.
Background. Streptomycetes were and remain a source of new antimicrobial compounds of various nature. A new wave of interest in such research is associated with the possibilities of applied genomics to reveal the hidden biosynthetic potential of streptomycetes, and therefore the discovery of new practically valuable antimicrobial products. The problem of antibiotic resistance of pathogens can be solved by creating compositions of active substances of different nature to overcome the protective mechanisms of pathogens. Objective. The purpose of the work was to establish and evaluate the antimicrobial and cytotoxic characteristics of the new antibiotic streptofungin, synthesized by Streptomyces albus 2435 (CMIM-S-668) and its mutant strains 2435/М, UN44, 4S, US101, AE6, 105, 80/5. Methods. To establish the characteristics of streptofungin, the antagonistic activity of selected S. albus producer strains was determined (by the radial streak method), the minimum inhibitory concentration of the antibiotic (by the serial dilution method), and cytotoxicity was determined by the MTT test with resazurin. Results. The activity of the antibiotic streptofungin against Candida fungi (C. albicans, C. utilis) is shown. Minimum inhibitory concentrations of streptofungin were determined for C. albicans ATCC 10231 (10 μg/ml), B. subtilis ATCC 6633 (200 μg/ml) and P. aeruginosa ATCC 9027 (500 μg/ml). According to the resazurin test, streptofungin does not show a cytotoxic effect in a wide range of concentrations from 2.5 to 500 μg/ml, and therefore can be considered potentially permissible for humans and animals in the studied concentrations. Conclusions. The antagonistic activity of mutant strains of S. albus culture is due to the action of a complex of antimicrobial products that have a different antimicrobial spectrum and mechanism of action. The obtained results give reasons to consider streptofungin as a promising pharmaceutical substance with antifungal action, as well as to consider the possibility of its combination with the bacteriolytic enzyme complex of the same culture for the development of an antimicrobial agent with a wide spectrum of action.
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Arterial Blood Oxygen Saturation and Its Dynamics Due to the Environmental Electromagnetic Conditions
(Igor Sikorsky Kyiv Polytechnic Institute, 2018) Mamilov, S. O.; Gorgo, Yu. P.; Yesman, S. S.; Vácziová, M.; Prigancová, A.; Pet'ko, B.
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Aspects of Lyophilization of Cardiac Bioimplant
(Igor Sikorsky Kyiv Polytechnic Institute, 2021) Shchotkina, N. V.
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Assessing the ethanologenic potential of xylose-fermenting yeasts Scheffersomyces stipitis UCM Y-2810
(Igor Sikorsky Kyiv Polytechnic Institute, 2025) Hretskyi, I.; Mokrousova, O.; Oleshko, A.; Budyakova, O.; Antonenko, D.; Fomina, M.
Background. Enhancing the efficiency of second-generation (2G) bioethanol production from lignocellulosic biomass is crucial for advancing sustainable biofuel technologies. However, the conversion of biomass into 2G bioethanol faces substantial challenges, necessitating a comprehensive investigation of microbial agents. Objective. To evaluate the effect of glucose and xylose concentrations, as well as cultivation duration, on the efficiency of ethanologenesis using the model organism Scheffersomyces stipitis UCM Y-2810, and to determine the optimal conditions for achieving maximum ethanol yield. Methods. The effects of glucose and xylose concentrations and cultivation time on ethanologenesis efficiency were evaluated using S. stipitis UCM Y-2810 as a model organism. The experimental design included three levels of factors: xylose concentration (3, 16.5, and 30 g/l), glucose concentration (1, 5.5, and 10 g/l), and cultivation durations (1, 2, and 3 days). Statistical analysis of the experimental data was conducted using a three-factor, three-level Box–Behnken design. Results. Under submerged cultivation of the strain of S. stipitis UCM Y-2810 in model media, optimization of the ethanologenesis process resulted in a maximum ethanol yield of 7.74 g/l. The optimal conditions for this yield were identified as follows: xylose concentration of 16.5 g/l, glucose concentration of 7.75 g/l, and a cultivation time of 3 days. Conclusions. The application of the Box–Behnken design revealed that the statistically significant factors influencing ethanologenesis efficiency were xylose concentration, yeast cultivation duration, and the linearquadratic interaction between these two factors.
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Attenuation of Paraquat-Induced Nephrotoxicity and Dysfunction in Male Wistar Albino Rats
(Igor Sikorsky Kyiv Polytechnic Institute, 2020) Ujowundu, C. O.; Anaba, P. I.; Ulinasombu, N. B.; Ujowundu, F. N.; Igwe, K. O.; Ogbuagu, H. D.
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Automated Assessment of a Students Circulatory System Functional State Using Martine's Test
(Igor Sikorsky Kyiv Polytechnic Institute, 2018) Voinyk, B. A.; Borisova, G. V.; Umanets, V. S.; Boiko, G. L.; Pavlov, A. V.; Nastenko, Ie. A.
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Bioinformatic Analysis of the Genetic Mechanism of Biomineralization of Biogenic Magnetic Nanoparticles in Bacteria Capable of Tumor-Specific Accumulation
(Igor Sikorsky Kyiv Polytechnic Institute, 2022) Gorobets, S.V.; Gorobets, O.Yu.; Kovalova, S.О.
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Biological Evaluation of Medical Devices in the Form of Suppositories for Rectal and Vaginal Use
(Igor Sikorsky Kyiv Polytechnic Institute, 2021) Dmytrenko, О. V.; Arkhypova, М. А.; Starosyla, D. B.; Rybalko, S. L.; Gevorkyan, M.; Galkin, A. Yu.
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Biological Risks of Using Non-Thermal Non-Ionizing Electromagnetic Fields
(Igor Sikorsky Kyiv Polytechnic Institute, 2020) Nizhelska, O. I.; Marynchenko, L. V.; Piasetskyi, V. I.
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Biomedical Engineering Education: Ukrainian Trajectory Through the Prism of World Experience. Status and Perspectives
(Igor Sikorsky Kyiv Polytechnic Institute, 2021) Galkin, Alexander; Галкін, Олександр
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Biosafety aspects of hybridoma technology: nature of risks and approaches to their management
(Igor Sikorsky Kyiv Polytechnic Institute, 2025) Shevchuk, Kateryna M.; Baranovska, Anastasia V.; Chernetskyi, Andrii S.; Shchotkina, Nataliia V.; Besarab, Alexander B.
This study investigates the biosafety aspects of hybridoma technology, focusing on the identification and management of associated risks. Monoclonal antibodies, essential tools in immunology, biotechnology, and medicine, are primarily produced through hybridoma technology. This process involves fusing B lymphocytes from immunized animals with myeloma cells to create hybridomas, which are then cultured to produce spe-cific antibodies. The research highlights significant contamination risks, particularly from rodent-borne virus-es and other pathogens, during both in vivoand in vitrocultivation. It systematically analyzes existing strate-gies for identifying and mitigating these risks at various stages of monoclonal antibody production, including hybridoma identification, cell fusion, and antibody purification. The study underscores the importance of stringent biosafety protocols and optimized purification methodologies to ensure the production of high-quality, contaminant-free monoclonal antibodies. Additionally, it emphasizes the necessity of comprehensive risk assessments and the implementation of advanced contamination control systems in laboratories. The conclusions drawn from this study provide valuable insights into enhancing the safety and efficacy of monoc-lonal antibody production. By addressing these biosafety concerns, the research supports the widespread ap-plication of monoclonal antibodies in scientific and medical fields, ensuring their reliability and effectiveness in various diagnostic and therapeutic contexts.
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Biosafety Management: Emphasis on Medicine, Pharmacy, and Biotechnology
(Igor Sikorsky Kyiv Polytechnic Institute, 2025) Galkin, Alexander
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Characterization of Placental Mesenchymal Stem Cells Spheroids after Generation, Hypothermic and Subnormothermic Storage
(Igor Sikorsky Kyiv Polytechnic Institute, 2019) Prokopiuk, V. Yu.; Hloba, N. S.; Prokopiuk, O. S.; Schedrov, A. O.; Musatova, I. B.

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