Determination of patulin in apple puree using LCMS with triple quadrupole detector
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Дата
2022
Науковий керівник
Назва журналу
Номер ISSN
Назва тому
Видавець
КПІ ім. Ігоря Сікорського; ТОВ «Українська водна спілка»
Анотація
Patulin is a high-hazardous mycotoxin formed in apples affected by the fungus. This mycotoxin,
when ingested, has the irreversible effect of deteriorating health. Most often, patulin enters the
human body through processed apple products or baby food. The countries of the European Union
have decided to reduce the level of the maximum residue level of patulin in apples to the lowest that
can be measured by analytical instruments. Therefore, the aim of this work is to develop a rapid
and sensitive method of patulin determination for the lowest level detection allowed by European
law.
In this work, patulin was artificially added to apple puree at the level of 10 g/kg for further
detection. The analysis was performed on an LC-MS/MS system with electrospray ionization type
and a column based on modified silica. The mobile phase used in the analysis was ultrapure water
and acetonitrile with the addition of formic acid. Measurement of the quantity of PAT in the sample
was performed using MRM transitions. Subsequently, there was a problem of significant inclusion
of the matrix on the spectrum, but the problem was solved by using cartridges for cleaning of
matrix impurities. As a result, we achieved a successful and accurate result at level of 10 g/kg. A
calibration curve was felicitously constructed for five different concentrations. The most important
parameters of validation of the method use were determined – reproducibility, repeatability,
recovery and linearity. The final result of this work is the development and implementation of a
rapid, cost-effective and suitable method for determining patulin at the level of the maximum
residue level.
Опис
Ключові слова
apple puree, liquid chromatography, mass spectrometry, mycotoxin, patulin, яблучне пюре, рідинна хроматографія, мас-спектрометрія, мікотоксин, патулін
Бібліографічний опис
Determination of patulin in apple puree using LCMS with triple quadrupole detector / M. Kuptsova, A. Shchepak, A. Slis, T. Solodovnik // Вода і водоочисні технології. Науково-технічні вісті. – 2022. – № 1(32). – С. 3-9.